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KMID : 1041219790210010021
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Journal of Sericultural and Entomological Science
1979 Volume.21 No. 1 p.21 ~ p.28
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Studies on the Rearing with Artificial Diet in the Silkworm, Bombyx mori L. -Electrophoretic Sepatarion of Esterase or Phosphatase along the Growth of Larvae-
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Kim Joo-Up
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Abstract
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The Electrophoretic separation in agarose gel on the esterase and acid phosphatase of blood, midgut and silk gland was carried out with 2 original variginal varieties and 7 F hybrids. 1. The midgut of larvae fed on mulberry leaves showed one or two more esterase bands than that of larvae fed on artificial diet. 2. The midgut of C 15 larvae being excellently respondent to artificial diet showed one or two more esterase bands than that of larvae being bad respondent to artificial diet. 3. Electrophoretic separation of esterase bands appeared to be greatly different among newly hatched larvae, 1st and 2nd install larvae of Fhybrids. However the difference among the silkworm varieties was not recognized. 4. According to the change in rearing temperature, the number of the active band of midgut esterase was varied. At the temperature of 28 5 active esterase bands were found. At temperature of 35 4 bands were noted at 3rd install and 6 or 7 bands at 4th instar. 5. No similar esterase bands conld be found among midgut, blood and silkgland. There are five esterase bands in the midgut, one in blood and three in silkgland. 6. There was rather small difference in acid phosphatase types of midgut and blood according to varieties and rearing temperature. No active band was shown in silkgland. In midgut, there was one acid phosphatase band at 3rd install, two at 4th instar and three at 5th instar. In blood, One active band at 3rd or 4th instar and three bands at 5th inatar were detected.
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